dara_assembly.fasta

Assembly of Darapsa myron from Genbank SRA accession #SRR1002986, using multiple kmers (13,23,33,43,63) with SOAPdenovo-Trans v1.01. Different Kmer assemblies were combined with cd-hit-est and process with the fastx toolkit. See SOAP_assembly.qsub for the command used for this assembly.本研究依托GenBank序列读取存档（Sequence Read Archive，SRA）登录号SRR1002986中的测序数据，对五叶地锦天蛾（Darapsa myron）进行转录组组装：采用SOAPdenovo-Trans v1.01工具，设置13、23、33、43、63共5个Kmer（k-mer）参数开展组装；随后通过cd-hit-est工具合并不同Kmer参数下得到的组装结果，并借助fastx工具包完成后续数据处理。本次组装所使用的命令可查阅SOAP_assembly.qsub文件。