Protein components of an internal core.

(A) Protein profiles of the core fraction. The cells were treated by Triton X-100, fractionated by sucrose-gradient centrifugation, subjected to SDS-PAGE, and stained by CBB. Gel images of 12% and 8.75% polyacrylamide are shown in the left and right panels, respectively. The amount of fraction applied to each lane was adjusted to derive from the same number of cells. The molecular mass is shown on the left. The protein bands identified by PMF are marked by an arrow with the gene ID (MPN number) and the annotation [13]. The asterisks indicate the bands that were not identified by PMF. Gray boxes highlight the identical protein bands identified from the gels with both acrylamide concentrations. The gene ID and annotations are colored according to their categories (see the text). (B) Cell images labeled by EYFP for MPN066, MPN332, and MPN387. Phase-contrast and fluorescence images are merged. The cell images labeled for other proteins are shown in S2 Fig. (C) ORFs encoding the components of the attachment organelle.