RNA-seq of cultured prefrontal cortex brain tissue from C57BL/6 mouse

FGF2 plays important roles in neural progentor cells prolifration and differency, however, the effects of FGF2 on neuronal cell culture are controversial and its effects on human embryonic prefrontal cortex tissues after culture have not been studied, therefore, we firstly investigated the effect of FGF2 on human embryonic cortex tissue and repeated with P0 mice embryonic prefrontal tissues after culture. Overall design: We performed RNA-seq on P0 mouse cultured brain tissue treated with or without FGF2. Prefrontal cortex brain cells were harvested as describrd in P0 mouse cortical brain neurons in vitro culture. 1-2 million cells were used for library construction.