Concentration and δ15N of amino acids in size-fractionated particles from the eastern tropical North Pacific ODZ

This dataset includes the concentration and δ15N of bulk N and amino acids in size-fractionated particles. Particle samples were collected aboard the R/V Sally Ride (cruise SR2011) from December 23 – 30, 2020 with a McLane large volume pumping system (WTS-LV). These data assess the N sources utilized by different phytoplankton communities inhabiting the two chlorophyll maxima and the transformation of particulate organic matter within the oxygen-deficient zone., Two particle fractions were collected at five stations in the eastern tropical North Pacific region. At each station, seawater was filtered sequentially through a 53 µm Nitex mesh and then a pre-combusted 142-mm-diameter glass fiber filter (GF-75: 0.3 µm retention size; or GF/F: 0.7 µm nominal retention size) with the McLane pump in situ. These filters were promptly frozen upon retrieval.Each 53 µm Nitex mesh was immersed in approximately 100 ml of MilliQ water and subjected to a 5-minute sonication. The solution was filtered onto pre-combusted 47-mm glass fiber filters (GF/F, 0.7 µm pore size), which retain the > 53 µm particle fraction. The GF-75 and GF/F filters (0.3 or 0.7 – 53 µm fractions) were subsampled, packed in tin capsules, and analyzed for concentration and δ15N of bulk materials by an elemental analyzer isotope-ratio mass spectrometer at the UC Davis Stable Isotope Facility. Bulk δ15N analysis was not performed for the > 53 µm fraction due to the limited amount of co..., , # Concentration and δ15N of amino acids in size-fractionated particles from the eastern tropical North Pacific ODZ [https://doi.org/10.5061/dryad.prr4xgxxf](https://doi.org/10.5061/dryad.prr4xgxxf) ## Description of the data and file structure This dataset includes the concentration and δ15N of bulk N and amino acids in size-fractionated particles. Two particle fractions were collected aboard the R/V Sally Ride (cruise SR2011) from December 23 – 30, 2020 at five stations with a McLane large volume pumping system (WTS-LV). These data assess the N sources utilized by different phytoplankton communities inhabiting the two chlorophyll maxima and the transformation of particulate organic matter within the oxygen-deficient zone. Two particle fractions were collected at five stations in the eastern tropical North Pacific region. At each station, seawater was filtered sequentially through a 53 µm Nitex mesh and then a pre-combusted 142-mm-diameter glass fiber filter (GF-75: 0.3 µm retention ...本数据集涵盖分级粒径颗粒物（size-fractionated particles）中的总氮（bulk N）与氨基酸的浓度及δ¹⁵N同位素比值。颗粒物样品于2020年12月23日至30日期间，搭载“莎莉·赖德号”（R/V Sally Ride）科考船（航次SR2011），采用McLane大体积抽滤系统（WTS-LV）采集。本数据旨在解析栖息于两个叶绿素最大值层的不同浮游植物群落所利用的氮源，以及缺氧带内颗粒有机质的转化过程。 在东热带北太平洋区域的5个站位共采集两组颗粒物分级样品。每个站位的海水依次经53 μm Nitex网筛过滤，再通过预灼烧的142 mm直径玻璃纤维滤膜（GF-75：截留粒径0.3 μm；或GF/F：标称截留粒径0.7 μm）完成过滤，过滤过程采用原位McLane泵进行。回收滤膜后即刻进行冷冻保存。 将每张53 μm Nitex网筛浸入约100 mL MilliQ超纯水中，进行5分钟超声处理，随后将处理液过滤至预灼烧的47 mm直径玻璃纤维滤膜（GF/F，孔径0.7 μm），该滤膜可截留>53 μm的颗粒物组分。 对GF-75与GF/F滤膜（对应0.3/0.7 μm ~53 μm粒径组分）进行分样并封装入锡囊，随后由加州大学戴维斯分校稳定同位素实验室（UC Davis Stable Isotope Facility）的元素分析仪-同位素比值质谱联用系统，完成总物质的浓度与δ¹⁵N分析。由于>53 μm组分样品量有限，未对其开展总氮δ¹⁵N分析。 # 东热带北太平洋缺氧带分级粒径颗粒物的氨基酸浓度与δ¹⁵N比值 DOI: https://doi.org/10.5061/dryad.prr4xgxxf ## 数据与文件结构说明 本数据集包含分级粒径颗粒物中的总氮与氨基酸的浓度及δ¹⁵N同位素比值。2020年12月23日至30日，搭载“莎莉·赖德号”（R/V Sally Ride）科考船（航次SR2011），使用McLane大体积抽滤系统（WTS-LV），于东热带北太平洋区域的5个站位采集了两组颗粒物分级样品。每个站位的海水依次通过53 μm Nitex网筛，再经预灼烧的142 mm直径玻璃纤维滤膜（GF-75：截留粒径0.3 μm；或GF/F：标称截留粒径0.7 μm）进行原位过滤。回收滤膜后即刻冷冻保存。