JFRC2 Template Brain

This single female brain was generated by Arnim Jenett (Janelia Farm Research Campus) and is stained with the neuropil marker nc82. If you make use of this data, please cite: A GAL4-Driver Line Resource for Drosophila Neurobiology Arnim Jenett, Gerald M. Rubinemail, Teri-T.B. Ngo, David Shepherd, Christine Murphy, Heather Dionne, Barret D. Pfeiffer, Amanda Cavallaro, Donald Hall, Jennifer Jeter, Nirmala Iyer, Dona Fetter, Joanna H. Hausenfluck, Hanchuan Peng, Eric T. Trautman, Robert R. Svirskas, Eugene W. Myers, Zbigniew R. Iwinski, Yoshinori Aso, Gina M. DePasquale, Adrianne Enos, Phuson Hulamm, Shing Chun Benny Lam, Hsing-Hsi Li, Todd R. Laverty, Fuhui Long, Lei Qu, Sean D. Murphy, Konrad Rokicki, Todd Safford, Kshiti Shaw, Julie H. Simpson, Allison Sowell, Susana Tae, Yang Yu, Christopher T. Zugates http://dx.doi.org/10.1016/j.celrep.2012.09.011 The original image had an XY resolution of 0.622088 microns × 0.622088 microns and a 1 micron slice spacing. It was then upsampled in Z to give isotropic voxels. When received from Janelia Farm the image had no spatial calibration and was in tif format. The image was opened in Fiji/ImageJ and the voxel size set to 0.622088 microns × 0.622088 microns × 0.622088 microns, based on a personal communication from Arnim Jenett and inspection of the image data associated with http://dx.doi.org/10.1016/j.celrep.2012.09.011 that is available at http://flweb.janelia.org/cgi-bin/flew.cgi. The image was then saved in gzip encoded nrrd format using the ImageJ Nrrd_Writer plugin.该单个雌性大脑样本由阿尔尼姆·詹内特（Arnim Jenett，贾内利亚农场研究园区）生成，使用神经纤维丛标记物（neuropil marker）nc82进行染色。若您使用本数据集，请引用以下文献：《用于果蝇神经生物学的GAL4驱动系资源》（A GAL4-Driver Line Resource for Drosophila Neurobiology），作者包括Arnim Jenett、Gerald M. Rubinemail、Teri-T.B. Ngo、David Shepherd、Christine Murphy、Heather Dionne、Barret D. Pfeiffer、Amanda Cavallaro、Donald Hall、Jennifer Jeter、Nirmala Iyer、Dona Fetter、Joanna H. Hausenfluck、Hanchuan Peng、Eric T. Trautman、Robert R. Svirskas、Eugene W. Myers、Zbigniew R. Iwinski、Yoshinori Aso、Gina M. DePasquale、Adrianne Enos、Phuson Hulamm、Shing Chun Benny Lam、Hsing-Hsi Li、Todd R. Laverty、Fuhui Long、Lei Qu、Sean D. Murphy、Konrad Rokicki、Todd Safford、Kshiti Shaw、Julie H. Simpson、Allison Sowell、Susana Tae、Yang Yu、Christopher T. Zugates，DOI: 10.1016/j.celrep.2012.09.011。原始图像的XY轴分辨率为0.622088微米×0.622088微米，切片间距为1微米，随后对其进行Z轴上采样以获得各向同性体素（isotropic voxels）。从贾内利亚农场获取该图像时，其未进行空间校准，且格式为TIFF（Tagged Image File Format）。研究人员通过Fiji/ImageJ软件打开该图像，并根据阿尔尼姆·詹内特的个人沟通以及对DOI: 10.1016/j.celrep.2012.09.011关联图像数据（可于http://flweb.janelia.org/cgi-bin/flew.cgi获取）的检视，将图像体素尺寸设置为0.622088微米×0.622088微米×0.622088微米。随后通过ImageJ的Nrrd_Writer插件，将图像保存为gzip压缩编码的NRRD格式。