Porewater and microbial community properties of permeable riverbed sediments across the south of England, 2018

This dataset contains in-stream measurements of sediment porewater nutrients, nitrification rates (and the fraction which is either fully oxidised to nitrate or reduced to N2 gas), and the abundance of microbial 16S rRNA and specific N-cycling genes and transcripts. Sediments were sampled in winter (February 2018) and summer (July 2018), from 12 UK rivers with permeable beds (sand or chalk geology) and a gradient of P concentrations, in the Hampshire Avon catchment, Kent, and Essex. Methods included measurements of porewater nutrients using Skalar SAN++AutoAnalyser, nitrification rates from in-situ ‘push-pull’ injections of 15N-labelled ammonia and -nitrite, and sediment microbial gene and transcript abundance by DNA extraction and qPCR. The work was supported by the Natural Environment Research Council (Grants NE/P01142X/1, NE/P011624/1; A new dynamic for Phosphorus in RIverbed Nitrogen Cycling - PRINCe Full details about this dataset can be found at https://doi.org/10.5285/d432d96c-7aff-45a5-9d4b-37e4065afdd7

本数据集收录了沉积物孔隙水中的营养物质测量值、硝化速率（以及完全氧化为硝酸盐或还原为氮气N2的比率），以及微生物16S rRNA和特定氮循环基因及转录本的丰度。沉积物样本采集于冬季（2018年2月）和夏季（2018年7月），地点为12条具有渗透性河床（沙质或石灰岩地质）的英国河流，这些河流位于汉普郡阿文流域、肯特郡和埃塞克斯郡，P浓度梯度各异。研究方法包括使用Skalar SAN++AutoAnalyser进行孔隙水营养物质测量、通过原位15N标记的氨和亚硝酸盐的‘推-拉’注射法测定硝化速率，以及通过DNA提取和qPCR技术检测沉积物微生物基因和转录本的丰度。该研究得到了自然环境研究委员会（NE/P01142X/1，NE/P011624/1；磷在河床氮循环中的新动态——PRINCe）的资助。关于本数据集的详细信息，请参阅https://doi.org/10.5285/d432d96c-7aff-45a5-9d4b-37e4065afdd7。