FIGURE 3
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Original confocal images for Figure 3 Fig 3a-r Confocal images for Serpinb3a+/+ (a-c; g-i, m-o) and Serpinb3a-/- (d-f; j-l; p-r) FIECS treated with 100% DPBS (a-f), STS (g-l) or 25% DPBS (m-r) and stained with Hoescht 33342 (C01), AnnexinV (C02) and propidium iodide (C03) Fig 3u-x Confocal images for Serpinb3a+/+ (u, w) and Serpinb3a-/- (v, x) FIECS treated with STS + zVAD.fmk in the presence of DMSO (u, v) or necrostatin-1 (w, x) and stained with Hoescht 33342 (C03) and propidium iodide (C02)
图3的共聚焦显微成像原始图像 图3a-r:针对Serpinb3a基因野生型(Serpinb3a+/+,对应a-c、g-i、m-o组)与Serpinb3a基因敲除型(Serpinb3a-/-,对应d-f、j-l、p-r组)的胎鼠肠上皮细胞单层(FIECS),分别经100%杜氏磷酸盐缓冲液(Dulbecco's Phosphate-Buffered Saline,DPBS,a-f组)、星形孢菌素(STS,g-l组)或25% DPBS(m-r组)处理后,以赫斯特33342(Hoescht 33342,C01)、膜联蛋白V(AnnexinV,C02)与碘化丙啶(propidium iodide,C03)完成染色。 图3u-x:针对Serpinb3a+/+(u、w组)与Serpinb3a-/-(v、x组)的FIECS,在二甲基亚砜(DMSO)或坏死素-1(necrostatin-1)存在的条件下,经STS与zVAD.fmk联合处理后,采用赫斯特33342(Hoescht 33342,C03)与碘化丙啶(propidium iodide,C02)进行染色。




