Summary table of qPCR, ddPCR and cdPCR performance for MON810 detection and quantification.

qPCR: data produced in this study, or obtained from the literature, when indicated [18], [22] ddPCR: data produced in this study. cdPCR: data produced on a BioMark System (Fluidigm, South San Francisco) using the 12.765 digital arrays (Fluidigm) and obtained from the literature [11], [18], [19]. Repeatability through the dynamic range: assessed through the coefficient of variation (Cv) of the target copy numbers or the MON810 content between repeats. Trueness: assessed through the calculation of the bias between the MON810 content measured and the target MON810 content. * For our study, trueness is indicated only when qPCR and ddPCR results could be compared to a third, independent value (obtained from the CRM provider or proficiency test organizer). Time for results/96 well plate: Total time needed from DNA pipetting to the analysis of the results; reaction mixes are already prepared. Price/sample if 96-well plate: Price based on material and reagent costs available at NIB, including labor cost. N.A.: not evaluated.