Gene expression profile at single cell level of injured enthesis at 7 day post repair operation from wild type and Nlrp3 knockout mice

The repair of injured enthesis remains clinically challenging in sports medicine. We have proved that the activation of Nlrp3 inflammasomes suppressed enthesis regeneration. So we used single cell RNA-seq to uncover the mechanisms by which Nlrp3 inflammasomes modulate enthesis regeneration Enthesis at 7 day post injury and repair from wild type and Nlrp3 -/- mice were carefully harvested under surgical microscope and pooled together. Single-cell RNA sequencing was used to analyze