Supplementing high-fibre olive pomace and multi-enzyme to broiler chicken's diet can improve health and performance

This study assessed the effects of high-fibre olive pomace (OP) and exogenous multi-enzyme (phytase, cellulase, and xylanase) on broiler chickens' growth performance, internal organs, intestinal morphology, blood traits, lipid profile of serum, and health. 300 one-day-old Ross 308 chicks were randomly divided into six groups using a 2 × 3 factorial design with 0 and 0.5g/kg multi-enzyme and 0, 5, and 10% olive pomace. The diet was primarily based on corn and soybean meal. The experiment was carried out in three stages: starter, grower, and finisher. The results indicated that the inclusion of OP at both 5% and 10% levels did not negatively impact broilers' performance (P>0.05). However, supplementing the diet with multi-enzyme significantly increased feed consumption, body weight, and weight gain (P<0.05). Furthermore, chickens offered with 10% OP + multi-enzyme exhibited the best performance compared to other experimental groups. The mortality rate also showed a non-significant decline of almost 5% (P>0.05). Additionally, administration of OP and multi-enzyme or a combination of them to the broilers' diet improved serum lipid profile and liver enzyme activity (P<0.05) and did not affect the relative weight and length of internal organs, and intestinal histomorphology (P>0.05). In conclusion, simultaneous use of multi-enzyme and diet containing olive pomace could improve the serum lipid profile, liver enzyme activity, and overall health without adverse effect on broilers performance.本研究评估了高纤维橄榄果渣（olive pomace, OP）与外源复合酶（植酸酶、纤维素酶、木聚糖酶）对肉鸡生长性能、内脏器官、肠道形态、血液指标、血清脂质谱及健康状况的影响。选取300只1日龄罗斯308（Ross 308）肉鸡雏鸡，采用2×3因子试验设计随机分为6组：复合酶添加水平设为0与0.5g/kg，橄榄果渣添加水平设为0、5%及10%。试验日粮以玉米和豆粕为主要原料，全程分为育雏期、育成期与育肥期三个阶段。结果表明，添加5%与10%浓度的橄榄果渣均未对肉鸡生长性能产生负面影响（P>0.05）。然而，日粮中补充复合酶可显著提升肉鸡采食量、体重及体增重（P<0.05）。进一步分析显示，饲喂10%橄榄果渣+复合酶组的肉鸡各项性能均优于其余试验组。试验组死亡率亦出现约5%的非显著性下降（P>0.05）。此外，在日粮中添加橄榄果渣、复合酶或二者联合使用，均可改善肉鸡血清脂质谱与肝脏酶活性（P<0.05），且未对内脏器官相对重量、长度及肠道组织形态学指标产生显著影响（P>0.05）。综上，复合酶与含橄榄果渣日粮联合使用，可在不影响肉鸡生长性能的前提下，改善其血清脂质谱、肝脏酶活性及整体健康状况。