3-way-aligned-orthologous-segments

This FASTA-formatted file is a series of 56 sequence alignments. Each alignment contains one sequence from each frog transcriptome studied. The sequences were aligned at the protein level using MUSCLE (Edgar 2004) and then converted to nucleotide alignments. Annotation information for each set of contigs can be found in the two annotation.xls spreadsheets. This file provides the expected amplicons from each reference sequence for the primers listed in conserved-primer-candidates-for-orthologous-segments.xls, for the purposes of guiding the selection of sequences that may be useful for a given population-genetic or phylogenetic study.本文件为FASTA格式（FASTA），包含56组序列比对结果。每组比对结果均包含本次研究涉及的所有蛙类转录组各一条序列。序列首先在蛋白质层面使用MUSCLE软件（Edgar等人，2004）完成比对，随后转换为核苷酸序列比对。各组重叠群（contigs）的注释信息可在两份annotation.xls电子表格中获取。本文件可提供针对conserved-primer-candidates-for-orthologous-segments.xls中所列引物，各参考序列对应的预期扩增子，用于指导筛选适用于特定群体遗传学或系统发育研究的序列。