Selecting for altered substrate specificity reveals the evolutionary flexibility of ATP-binding cassette transporters, Srikant S et al

This is the accompanying data for the Current Biology article titled "Selecting for altered substrate specificity reveals the evolutionary flexibility of ATP-binding cassette transporters" authored by Sriram Srikant, Rachelle Gaudet and Andrew W Murray (https://doi.org/10.1016/j.cub.2020.02.077). The sequence alignment and homology models presented here support the inferences made in our manuscript. The autocrine data presented here is the raw data supporting our figures. Data index: MendeleySourceDataFile1.fasta | Alignment of fungal pheromone ABC exporters focused on Y. lipolytica Ste6. MendeleySourceDataFile2.pdb | Homology model of YlSte6 made based on inward-open LTC4-bound BtMRP1 (PDB:5UJA). MendeleySourceDataFile3.pdb | Homology model of YlSte6 made based on outward-open ATP-bound BtMRP1 (PDB:6BHU). MendeleySourceDataFile4.xlsx | Autocrine data of ScSte6, YlSte6, selected clones and mutational combinations of YlSte6 used to test additive model (Figure 6). MendeleySourceDataFile5.csv | Autocrine data of all selected clones tested by flow cytometry (Figure S2 and S3).本数据集为发表于《当代生物学》（Current Biology）、题为《筛选改变的底物特异性揭示ATP结合盒转运蛋白（ATP-binding cassette transporters）的进化灵活性》的配套研究数据，作者为Sriram Srikant、Rachelle Gaudet与Andrew W Murray（DOI: 10.1016/j.cub.2020.02.077）。 本文提供的序列比对结果与同源建模模型，可支撑论文中的各项推论；文中附带的自分泌原始实验数据，可对应支撑论文中的各类图表内容。 数据文件索引如下： MendeleySourceDataFile1.fasta：聚焦解脂耶氏酵母（Y. lipolytica）Ste6蛋白的真菌信息素ATP结合盒转运蛋白外排泵序列比对文件 MendeleySourceDataFile2.pdb：基于向内开放构象结合白三烯C4（LTC4）的牛源多药耐药相关蛋白1（BtMRP1，PDB:5UJA）构建的YlSte6同源建模模型文件 MendeleySourceDataFile3.pdb：基于向外开放构象结合ATP的牛源多药耐药相关蛋白1（BtMRP1，PDB:6BHU）构建的YlSte6同源建模模型文件 MendeleySourceDataFile4.xlsx：酿酒酵母Ste6（ScSte6）、解脂耶氏酵母Ste6（YlSte6）、筛选获得的克隆株以及用于验证加性模型的YlSte6突变组合的自分泌实验数据文件（对应论文图6） MendeleySourceDataFile5.csv：所有经流式细胞术检测的筛选克隆株的自分泌实验数据文件（对应论文补充图S2与S3）