Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 22/01/2005 to 27/01/2005 (Dumont d'Urville - Hobart)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 22/01/2005 to 27/01/2005 on a trip from Dumont D'Urville to Hobart.

Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).

Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于“阿斯托拉贝号”考察船（RV L'Astrolabe，平台编码：FHZI），采集时段为2005年1月22日至2005年1月27日，航次为从迪蒙·迪维尔站（Dumont D'Urville）前往霍巴特的航行任务。

光系统II（PSII）的最大光化学效率，亦称为PSII最大量子产额（Fv/Fm），已成为浮游植物研究中应用最为广泛的荧光参数之一。该指标表征潜在光化学效率，即光合装置捕获的光能被用于光化学反应的概率。研究表明，Fv/Fm可对水体理化性质的变化产生即时响应，常被用作浮游植物整体健康状态与生理活性的诊断指标。结合光系统II吸收截面面积与叶绿素浓度数据，该指标可用于海洋初级生产力的测定（Cheah等人，2011年，《深海研究》（Deep Sea Research））。

实验采用船舶洁净海水管路连续抽取3米深度的海水。在暗适应状态（需经15分钟暗适应）下，以1分钟为间隔连续测量快速重复率（FRR）荧光产额；测量所用闪光序列包含两组：100个次饱和微闪光（闪光时长1.1 μs，闪光间隔2.8 μs）与20个弛豫微闪光（闪光时长1.1 μs，闪光间隔51.6 μs）。