TABLE 1 in Endophytic fungi associated with Coriaria nepalensis in an abandoned open-pit phosphate mine in Yunnan, P. R. China

TABLE 1. The PCR conditions and the primers used in this study.
LocusDefinitionPrimerAnnealing (PCR)ReferencesITS4ITSInternal transcribed spacera55 °C, 50 sbWhite et al. (1990)ITS5LR0RLSULarge subunit 28Sa55 °C, 50 sbVilgalys & Hester (1990)LR55Frpb2DNA-dependent RNA polymerase II largest subunita57 °C, 55 sbLiu et al. (1999)7cR728Ftef1-αTranslation elongation factor 1 alphaa56 °C, 50 sbZhang et al. (2021)986RBt2aBt2bO’Donnell & Cigelniktub2Beta-tubulin 2a52 °C, 50 sb(1997),T1Nosratabadi et al. (2018)T22
a Initial denaturation of 2 min at 94 °C, followed by 35 cycles of denaturation at 95 °C for 30 sec, annealing temperature and time for different genes are mentioned in Table 1. b 90 sec of extension at 72 °C and a final extension of 10 minutes at 72 °C.