BALB/c mice injected with iSPZ-loaded hepatocytes are protected against SPZ challenge.

BALB/c mice were injected (IS transfer) with 7×105PbiSPZ-infected or naïve BALB/c hepatocytes. Infected hepatocytes were obtained from BALB/c mice immunized with 106 ANKA wt iSPZ 2 h earlier. Recipient mice were then challenged with two different doses (2×103 and 5×103, respectively, A and B) of live PbSPZ one week later. Mice were considered protected if they remained parasite negative 2 weeks after challenge.