RNAseq of Burkholderia pseudomallei Bp82 Parent and deltaII2523 pellicle biofilms grown at 28C and 37C. Bp82 Parent and deltaII2523 RNAseq

In Burkholderia pseudomallei, the gene encoding for II2523 contains PAS and GGDEF domains and is responsible for thermoregulation of biofilm formation at 28C and 37C temperatures. A clean deletion mutant of II2523 in Burkholderia pseudomallei Bp82 (a Select Agent exempt strain) and the parental strain were grown as pellicle biofilms in 6-well culture plates at either 28C or 37C in biological triplicate. RNA was extracted from the pellicle biofilms after 24hr growth at the two temperatures and libraries were prepared for sequencing on an Illumina NextSeq500 flow cell. 12 samples in total as follows: deltaII2523 A,B,C -> 28C; deltaII2523 D,E,F -> 37C; Bp82 Parent A,B,C -> 28C; Bp82 Parent D,E,F -> 37C.