Example dataset that can be used for testing the EASI-FISH (Expansion-Assisted Iterative Fluorescence in situ hybridization) data analysis pipeline (https://github.com/JaneliaSciComp/multifish). Raw i
We used Escherichia coli transformed with pJKR-H-tetR plasmids encoding an inducible GFP gene as a model system to assess and optimize MGE-FISH on a confocal microscope. We designed FISH probes for th
The hybrid chain reaction (HCR), an isothermal and enzyme-free amplification strategy, has found extensive use in fluorescent in situ hybridization (FISH) assays. However, the existing HCRs are limite
Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination
Zip file containing individual tif files of blue (DAPI) green (FITC) and red (Alexa594) fluorescence taken on Zeiss or Nikon epifluorescence microscope. File name give Slide code and probe combination