LC-MS results of chemical modification of Aurora-A C288Dha and C275Dha.

aCompounds grouped by properties: polar uncharged (1–5), polar aromatic (6–9), hydrophobic (10–13), negatively charged (14–16), and positively charged (17–19). bReaction of Dha with 5000 eq. of thiol reagent, unless otherwise stated.cNot determined, likely to denature protein.dC275 cysteine to Dha reaction is incomplete, so unreacted cysteine is present in all final products.eNot determined for C275; results for C288 from previous work [18]. ‘Excellent conversion’ is defined as full reaction of Dha to the desired product, with no peaks observed by LC-MS to indicate unreacted Dha.