Using chemical genomics to study environmental toxins and mycoparasitic interaction

The attached data file is a list of yeast Saccharomyces cerevisiae sensitive/resistant deletion mutant strains in chemical genomics screens (homozygous profiling). Here, the following compounds were screened:

1. N-nitrosodimethylamine (NDMA).

2. N-nitrosodiethylamine (NDEA).

3. N-nitroso-N-methyl-4-aminobutyric acid (NMBA).

4. N,N-dimethylformamide (DMF).

5. Methylamine.

6. Ethylamine.

7. Formamide.

8. Formaldehyde.

9. 4-nitroquinoline-1-oxide (4NQO).

10. Ammonium sulfate.

11. Ascorbic acid*.

12. Ferulic acid*. *Added in cells treated with NDMA or NDEA; there is also control having only ascorbic and/or ferulic acid. Some compounds were screened more than once, and the dose and date for each analysis were included.

This repository is created for my thesis (“Understanding cellular response to drugs and toxins with yeast genomics tools” by Joseph Uchechukwu Ogbede) though the data is already published in “Ogbede, J.U., Giaever, G. & Nislow, C. A genome-wide portrait of pervasive drug contaminants. Sci Rep 11, 12487 (2021). https://doi.org/10.1038/s41598-021-917”. 

In the file name ‘Yeast-predation-screens’, the deletion mutants of Saccharomyces cerevisiae were competitively grown with Saccharomycopsis schoenii for 12 and 24 hrs. The data revealed several deletion mutants (hits) of S. cerevisiae that are sensitive or resistant when exposed to S. schoenii predation (mycoparasitic interaction). The data is being prepared (alongside wildtype S. cerevisiae vs S. schoenii interaction transcriptomics data) for publication.