1HNMR cerebrospinal fluid spectra image series
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Metabolic spectra of the dogs included in the study. The main chemical shift for each metabolite from left to right are: - Lactate: 4 and 1.3 ppm - Glucose: 3.8 and 3.4 ppm - Glutamate- Glutamine: 3.8 ppm - Myoinositol: 3.6 ppm - Taurine: 3.4 ppm - Choline: 3.2 ppm - Creatine: 3 ppm - N-acetyl aspartate: 1.8ppm Sample contamination with ethanol generates peaks in the spectrogram at 1.2 and 3.6 ppm, whith overlap with numerous metabolites including lactate, creatine, glutamate and glutamine. In Idiopathic epilepsy group, patients 1-5, 7 and 10 show this sample contamination. In Meningoencephalitis of unknown origin group, patients 5, 7-9, 10, 12-18, 21, 23 and 25-28 show this sample contamination. Sample contamination with sodium pentobarbital generates peaks in different shift but the 1.2 ppm peak is the most intense and interferes with lactate peak. Sodium pentobarbital contamination has been observed only in meningoencephalitis of unknown origin group in patients 7,8,16,17,25,26.
本研究纳入的犬只代谢波谱数据。各代谢物的主要化学位移(从左至右)依次为: - 乳酸盐(Lactate):4 ppm 与 1.3 ppm - 葡萄糖(Glucose):3.8 ppm 与 3.4 ppm - 谷氨酸-谷氨酰胺(Glutamate-Glutamine):3.8 ppm - 肌醇(Myoinositol):3.6 ppm - 牛磺酸(Taurine):3.4 ppm - 胆碱(Choline):3.2 ppm - 肌酸(Creatine):3 ppm - N-乙酰天门冬氨酸(N-acetyl aspartate):1.8 ppm 样本受乙醇污染时,将在波谱图的1.2 ppm与3.6 ppm位置生成峰值,该峰值与乳酸盐、肌酸、谷氨酸及谷氨酰胺等多种代谢物的峰值存在重叠。 在特发性癫痫组中,编号1至5、7及10号受试犬存在此类样本污染。 在未知病因脑膜脑炎组中,编号5、7至9、10、12至18、21、23及25至28号受试犬存在此类样本污染。 样本受戊巴比妥钠(Sodium pentobarbital)污染后,将在不同化学位移处生成峰值,其中1.2 ppm处的峰值强度最高,且会干扰乳酸盐的峰值信号。仅在未知病因脑膜脑炎组中观察到戊巴比妥钠污染情况,涉及受试犬编号7、8、16、17、25、26。




