Mus musculus intestinal epithelial MODE-K cells incubated with Heligmosomoides bakeri secreted extracellular vesicles (EVs)

Small RNAs are emerging as important molecules for cross-species communication. Thanks to available and affordable sequencing technologies it is now possible to sequence small RNAs (sRNA-Seq) present in samples of interacting organisms. A first step when analyzing sRNA-Seq of two interacting species is to determine which sequences are being produced by which organism. Due to their small size (18-30), small RNAs could easily map to both host and parasite genomes. Here we produced data for Mus musculus intestinal epithelial cells treated with Extracellular Vesicles (EV) produced by the parasitic nematode Heligmosomoides bakeri. Overall design: MODE-K cells were incubated with H. bakeri vesicles for 4 and 24 hours, cells were washed with PBS buffer before small RNA sequencing, three biological replicates were generated for each condition. Sequencing was performed on an Illumina HiSeq high output v4