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Pastor WGS vs IWGSC RefSeq v1.0 Genome Assembly

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Research Data Australia2024-12-14 收录
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https://researchdata.edu.au/pastor-wgs-vs-genome-assembly/1604028
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WGS reads of Pastor (http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x) were aligned against the IWGSC Triticum aestivum Chinese Spring RefSeq v1.0 genome assembly using Minimap2. These BAM files (.bam) and index files (.bam.bai) are provided together with a summary of read alignment coverage bigWig files (.bam.bw). SNP variants were called from these BAM files to generate VCF files (.bam.vcf.gz) and index files (.bam.vcf.gz.tbi) and are provided together with a summary of SNP density (SNPs per 10 kbp) bigWig files (.bam.vcf.w10000_s10000.bw). VCF files contain the following filter values and corresponding meaning: PASS = high quality (Q>=30) homozygous; Het = high quality (Q>=30) heterozygous; LowQualHom = low quality (Q

本数据集包含来自Pastor(http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x)的全基因组测序(Whole Genome Sequencing, WGS)读段,使用Minimap2将其比对至国际小麦基因组测序联盟(International Wheat Genome Sequencing Consortium, IWGSC)的普通小麦(Triticum aestivum)中国春参考基因组组装版本v1.0。本数据集附带BAM(Binary Alignment Map)格式比对文件(.bam)及其索引文件(.bam.bai),以及用于展示读段比对覆盖度的bigWig格式汇总文件(.bam.bw)。基于上述BAM格式比对文件调用单核苷酸多态性(Single Nucleotide Polymorphism, SNP)变异,生成VCF(Variant Call Format)格式变异文件(.bam.vcf.gz)及其索引文件(.bam.vcf.gz.tbi),同时附带用于展示SNP密度(每10千碱基对的SNP数量)的bigWig格式汇总文件(.bam.vcf.w10000_s10000.bw)。上述VCF格式变异文件包含以下过滤值及其对应含义:PASS=高质量(Q≥30)的纯合变异;Het=高质量(Q≥30)的杂合变异;LowQualHom=低质量(Q
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The University of Adelaide
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