Expression data of glutathione-fed Arabidopsis thaliana

To comprehensively investigate the effects of glutathione on the gene expression, the microarray analysis was performed in the glutathione-fed wild-type Arabidopsis thaliana. Wild-type Arabidopsis (ecotype Columbia-0) were fed with 1 mM oxidized glutathione (GSSG) and 2 mM reduced glutathione (GSH) for comparison at equal nitrogen equivalents. To examine the effects of glutathione other than nitrogen at equal nitrogen equivalents, plants were fed with 3 mM NH4NO3. Plants grown by water were used as a control. From the first week to the fifth week after sowing, a 25 ml of 1 mM oxidized glutathione (GSSG), 2 mM reduced glutathione (GSH) and 3 mM NH3NO4 aqueous solution per pot were applied by subirrigation once a week. Plants grown by water were used as control. The aerial parts including the shoot apical were harvested 3 weeks after sowing, and the second and third leaf blades from the top of the fully expanded leaves were harvested 4 and 5 weeks after sowing. Twelve samples. Two replicates for each samples.