Results of two-sided t-tests performed on adjusted fold change between adults and juveniles (left) and bivariate fits of adjusted fold change by estimated age (right).

For all t-tests, there were 13 degrees of freedom, and bivariate fits had 11 degrees of freedom. Fold change values were adjusted to the mean of all juvenile samples and also to differences in mean juvenile expression between batches (see Materials and Methods). Asterisks denote level of significance (*p≤0.05, **p≤0.01, ***p≤0.005).