Expression data from P14 Lgr5-2A-EGFP mouse uterus

Epithelial gland development within the uterine lining during prepubertal period is important to ensure successful gestation in adults. Lgr5 expression in uterus becomes largely restricted to the tips of developing glands after birth. These Lgr5 highly expressing cells function as stem cells during gland development. We used microarrays to detail the gene expression profilings and compare between Lgr5 highly and negatively expressing cells in developing uterus. Uteruses from Lgr5-2A-EGFP mice aged at P14 were used for RNA extraction and hybridization on Affymetrix microarrays. We sorted EGFP high and negative cells by FACS and proceeded for RNA extraction.