Summary of antigen selection and Th1 epitope prediction.

aSignal peptides and signal peptide type of each protein were predicted with software SignalP 4.0 or LipoP 1.0, which are available online (http://www.cbs.dtu.dk/services/SignalP-4.0 and http://www.cbs.dtu.dk/services/LipoP-1.0. Accessed 30 March 2013).bThe subcellular localization of each protein was predicted using PSORTb 3.0.2 or SOSUI-GramN, which is available online (http://www.psort.org/psortb/index.htmlandhttp://bp.nuap.nagoya-u.ac.jp/sosui/sosuigramn/sosuigramn_submit.html. Accessed 22 January 2013).SpI, signal peptide (signal peptidase I); EC, extracellular; OM, outer membrane; PP: periplasmic; C, cytoplasmic.