Bacillus subtilis BSH has a protective effect on salmonella infection by regulating the structure of intestinal flora in chickens.

Salmonellosis is a worldwide zoonotic disease, which poses a serious threat to the reproduction of livestock and poultry and the health of young animals. Probiotics have been paid more and more attention as a substitute for antibiotics, and Bacillus is one of them. In this study, the chicks infected with Salmonella were fed with feed supplemented with Bacillus BSH strain to observe the pathological changes of the liver, detect the number of viable bacteria in the liver and spleen, and record the death of the chicks. The results showed that BSH could reduce the pathological changes of the liver and the invasion of Salmonella into the 0liver and spleen of chicks. In addition, the survival rate of chicks in the BSH experimental group was 60%, while that in the infected control group was 26%, indicating that strain BSH had a protective effect on chicks infected with Salmonella. Finally, the fecal microflora of 9-day-old chicks was analyzed by 16SrRNA high-throughput sequencing. The results showed that salmonella infection could cause intestinal flora changes, while BSH could alleviate this change. In addition, BSH can also promote the proliferation of Lactobacillus salivarius in intestinal saliva. This study emphasized that Bacillus subtilis BSH has the effect of anti-salmonella infection in chickens and can be used as a candidate strain of microecological preparation.