Intracellular transcriptional level of Brucella genes obtained by qRT-PCR and analysis of gene functional characteristics.

aB. abortus ORFs listed are used locus tag of genes in B. abortus strain 9-941.bSubcellular locations were predicted by the PSORTb v.3.0 server. Available: http://www.psort.org/psortb/index.html. Accessed 10 December 2012.cFunctional characterization of the proteins was predicted by the software COGnitor. Available: http://www.ncbi.nlm.nih.gov/COG/old/xognitor.html. Accessed 10 December 2012. Functional categories: (1) Information storage and processing: (J: Translation, ribosomal structure and biogenesis; K: Transcription; L: DNA replication, recombination and repair); (2) Cellular processes: (D: Cell division and chromosome partitioning; O: Posttranslational modification, protein turnover, chaperones; M: Cell envelope biogenesis, outer membrane; P: Inorganic ion transport and metabolism; T: Signal transduction mechanisms); (3) Metabolism: (C: Energy production and conversion; E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism); (4) Poorly characterized: (R: General function prediction only; S: Function unknown).d-: No related COG.eResults are expressed as 2−ΔΔCt. Figures = 1 indicate that the gene is expressed similarly in both conditions, figures >1 indicate that the gene is over expressed in intracellular Brucella, and figures Brucella.