Genome-wide binding sites of Hypoxia inducible factor 1(HI1) in renal epithelial cells.

We report the high-throughput profiling of HIF1 in human kidney-2 cells (HK-2) under normoxia and 1% 24 hours hypoxia. By obtaining over two billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of HK-2 under normoxia and hypoxia. We found that HIF1 binds to both transcriptional starting sites and enhancer regions. This study provides novel insights into the epigenetic regulation of HIF1 in renal epithelial cells. We exposed HK-2 cells under 1% hypoxia for 24 hours. We collected mRNA of each samples and performed ChIP using HIF1 antibody. The ChIP DNA fragments were analyzed by NGS.