RNA Sequencing of peripheral blood mononuclear cells (PBMCs) isolated from epilepsy patients treated with vagus nerve stimulation and not treated with vagus nerve stimulation.

Purpose: To decipher the mechanisms responsible for the protective function of vagus nerve stimulation. Methods: Blood was collected from Control and Treated patients followed by isolation of PBMC'S. Total RNA was extracted and RNA sequencing was done following standard protocols Results: Detailed analysis of the transcriptomic data show that anti-inflammatory responses were upregulated in the treated samples. Conclusions: This study showed the protective role of vagus nerve stimulation in epilepsy patients. Overall design: Human PBMC's RNA seq of 4 control and treated 4 patients, using Illumina platform