Nasal 16S rRNA gene amplicons obtained from healthy and BRD-positive pen-mates Holstein steers.

Bovine respiratory disease (BRD) is an ongoing and vital problem in dairy and beef cattle. Thus, the objective of the study focuses on the characterization of the nasal microbiome of apparently healthy (n=75) and BRD-affected (n=58) pen-mates Holstein steers as a potential additional diagnostic tool to identify an animal with BRD. For the nasal microbiome analysis, the region V4 of the 16S rRNA gene was amplified and sequenced using Illumina MiSeq Sequencer.