Bulk RNA Barcoding and sequencing (BRB-seq) of Mock, ICV, and IDV infected airway epithelial cell cultures of human, porcine, and bovine origin at 33°C and 37°C

For the assessment of host response dynamics to ICV and IDV infections in human, porcine, and bovine airway epithelial cells at ambient temperature corresponding to the upper or lower respiratory tract. We performed a temporal transcriptome analysis on human, porcine, and bovine airway epithelial cell (AEC) cultures infected with ICV and IDV, as well as uninfected hAEC cultures, incubated either at 33°C or 37°C. AEC cultures from the different species were harvested at 24, 48 72, 96 hpi and processed for Bulk RNA Barcoding and sequencing (BRB-seq), which allows a rapid and sensitive genome-wide transcriptomic analysis in a highly multiplexed manner. Transcriptome data from human, procine, and bovine was obtained from a total of 3 biological donors for pairwise comparisons of ICV and IDV virus-infected to unexposed AEC cultures at respective time points and temperatures.