Oocytes formation in post-diapause Neocalanus flemingeri females from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13

These data are from a study on the formation of oocytes in post-diapause Neocalanus flemingeri females collected from depth in Prince William Sound in the Gulf of Alaska. Collections were made during two NGA LTER cruises. After sorting, females were incubated in flasks and removed for experimental incubations and imaging. Oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2’-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU labeled cells peaking at 72 hours following diapause termination. Cells labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. By limiting DNA replication to the initial phase, the females effectively separate oocyte production from oocyte provisioning.本数据集源自一项针对阿拉斯加湾威廉王子湾深海采集的滞育后费氏新哲水蚤（Neocalanus flemingeri）雌性个体卵母细胞形成过程的研究。数据采集依托两次NGA长期生态研究（Long-Term Ecological Research, LTER）航次完成。经分选后，雌性个体被置于烧瓶中培养，并分批取出用于后续实验培养与成像分析。针对滞育后雌性个体的卵母细胞生成过程（涉及卵巢与输卵管内的DNA复制），本研究通过5-乙炔基-2’-脱氧尿苷（EdU）标记培养开展了观测。结果显示，卵原细胞与卵母细胞均可掺入EdU，EdU标记细胞的数量在滞育终止后72小时达到峰值。EdU阳性标记细胞的数量在两周内维持较高水平，随后逐渐下降，至滞育后四周时已无标记信号可被检测到，该时间点距首次产卵集群的产出早3至4周。通过将DNA复制过程限定在初始阶段，雌性个体可有效区分卵母细胞生成与卵母细胞养分供给两个过程。