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Test data for Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view

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4TU.ResearchData2024-12-03 更新2026-04-23 收录
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https://data.4tu.nl/datasets/311fa522-2ce5-47f6-8674-10e001f7e909/1
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Test data for the software demo of the manuscript 'Calibration-free estimation of field dependent aberrations for single molecule localization microscopy across large fields of view'. This dataset contains the first 800 frames from the 3D microtuble dataset from Figure 2 of the manuscript. It is a test dataset for the Software Demo accompanying the manuscript.<br>It concerns raw data for Single Molecule Localization Microscopy of microtubules of HeLa cells over a large 97 x 97 μm field of view. The images were acquired with 2 cylindrical lenses at the Fourier plane of the microscope to introduce astigmatism. The data was acquired in order to demonstrate the estimation of field dependent aberrations from single molecule localization.

本数据集为论文《大视场单分子定位显微镜的场依赖像差无校准估计》配套软件演示的测试数据,取自该论文图2中的3D微管数据集的前800帧图像,是该论文配套软件演示专用的测试数据集。 本数据集包含97×97 μm大视场下的海拉细胞(HeLa cells)微管的单分子定位显微镜(Single Molecule Localization Microscopy)原始成像数据。成像过程中在显微镜的傅里叶平面加装两片柱面镜以引入像散,该数据集的采集旨在演示基于单分子定位的场依赖像差估计方法。
提供机构:
Droste, Isabel; Schuitema, Erik; van Werkhoven, Ben; Khan, Sajjad; Heldens, Stijn; Stallinga, Sjoerd; Lidke, Keith A.; Rieger, Bernd
创建时间:
2024-12-03
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