Effect of NF45 knock-down on whole gene expression in Beta-TC-6 cells, a pancreatic beta cell line

We found that knock down of NF90 and its binding factor, NF45, complex leads to retardation of growth in Beta-TC-6 cells, a pancreatic beta cell line. To uncover molecular mechanisms for the growth retardation in Beta-TC-6 cells depleted of NF90-NF45, we performed comprehensive analysis of gene expression using microarray. RNAs were extracted from Beta-TC-6 cells depleted of either NF45 or a non-targeting control. The RNAs were hybridized with GeneChip Mouse Gene 2.1 ST Array Strip which is an Affymetrix microarray