Identification of differentially expressed genes associated with endotracheal intubation-induced tracheal injury in a rabbit model of septic shock

To identify differentially expressed genes (DEGs) potentially involved in the development of endotracheal intubation-induced tracheal injury in septic shock. Overall design: Eighteen New Zealand white rabbits were randomized to control, septic shock model, and treatment (n = 6/group). The model and treatment groups were injected with lipopolysaccharide to induce septic shock. The treatment group was administered Ringer lactate with norepinephrine to maintain the normal blood pressure. All animals underwent cuffed endotracheal intubation for 2 h. The injured tracheal segment was harvested. RNA sequencing was performed to identify the DEGs, followed by bioinformatics analysis.