Genotyping-by-sequencing of several coastal and interior Douglas-fir (Pseudotsuga menziesii) provenances with ApeKI and HindIII as restriction enzymes

A genotyping-by-sequencing (GBS) experiment with 96 trees of several coastal and interior Douglas-fir (Pseudotsuga menziesii) provenances was performed. DNA of the trees was digested with two restriction enzymes (ApeKI and HindIII, double digestion). Fragmented and barcoded DNA were pooled and then sequenced on one lane of a HiSeq1000. Barcodes are: BA01 - CTCC, BA02 - TGCA, BA03 - ACTA, BA04 - CAGA, BA05 - AACT, BA06 - GCGT, BA07 - CGAT, BA08 - GTAA, BA09 - AGGC, BA10 - GATC, BA12 - TGCGA, BA13 - CGCTT, BA14 - TCACC, BA15 - CTAGC, BA16 - ACAAA, BA17 - TTCTC, BA18 - AGCCC, BA19 - GTATT, BA20 - CTGTA, BA21 - ACCGT, BA22 - GCTTA, BA23 - GGTGT, BA24 - AGGAT, BA25 - ATTGA, BA26 - CATCT, BA27 - CCTAC, BA28 - GAGGA, BA29 - GGAAC, BA30 - GTCAA, BA31 - TAATA, BA32 - TACAT, BA33 - TCGTT, BA34 - TTGTCA, BA35 - AATGCT, BA36 - TTACGA, BA37 - GGCTAT, BA38 - AATCGA, BA39 - CCGTAT, BA40 - TTAGCC, BA41 - GGCATA, BA42 - AAGCAT, BA43 - CTATGC, BA44 - TCCGCA, BA45 - AGTATC, BA46 - GAACCT, BA47 - CCTTGA, BA48 - TGGACC, BA49 - GAATTC, BA50 - ACTGAA, BA51 - AGCCTT, BA52 - CTGAGA, BA53 - GATACC, BA54 - CGACAT, BA55 - TTCAGC, BA56 - AGTCGGT, BA57 - CCTAAGA, BA58 - TTCGTGA, BA59 - ACGTGGT, BA60 - GGACAGT, BA61 - CACATGA, BA62 - TGTTCTA, BA63 - CTGAGGT, BA64 - GAAGTCA, BA65 - ACCGCAT, BA66 - CATTGGT, BA67 - ACCTAGA, BA68 - TGTCTCA, BA69 - ATCGGTT, BA70 - CCATGAA, BA71 - GTTCCTA, BA72 - TGAACCA, BA73 - ACTGATT, BA74 - TTCCGAA, BA75 - AGCTTGT, BA76 - CCACGCT, BA77 - CTTGAAT, BA78 - AACCACGT, BA79 - CTTGTTGA, BA80 - AGGTCGGT, BA81 - TAACGAGA, BA82 - GCCAACGT, BA83 - CTGTTGGA, BA84 - TGAATCGT, BA85 - GACCATGA, BA86 - CGTTAGGT, BA87 - ACCATAGA, BA88 - TGTTCTGA, BA89 - CTGGAGGT, BA90 - ACCACGTT, BA91 - GAACAATA, BA92 - CTTATGAA, BA93 - GCCACAAT, BA94 - CTGTGTTA, BA95 - TATAACGA, BA96 - GCACCATT, BA97 - CTTGGTAT