CTLA-4-Ig internalizes CD80 in fibroblast-like synoviocytes from chronic inflammatory arthritis mouse modelinflammatory arthritis mouse model

In this study, the therapeutic effect of CTLA-4-Ig in a mouse model of rheumtaoid arthritis, in which CD80 is expressed in chondrocytes and the synovial cells of joints. The therapeutic effect of CTLA-4-Ig appears to extend beyond the lymph nodes into the inflamed synovial compartment through the synergistic inactivation of T cells by the CD80 and PD-L1 axes. Isolated synovial cells were cultured in DMEM with 10% FBS and cultured at 37°C containing 5% CO2 for overnight. Abatacept (100 mg/ml), human IgG (100 mg/ml), and vehicle (DMEM with 10% FBS as a control) added and incubated at 37°C with 5% CO2 for 48 h.