Quantification of the PTP1B presence in dendritic protrusions/spines, presynaptic puncta, and synapses.
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Dendritic spines were morphologically visualized by F-actin staining with phalloidin and presynaptic puncta were recognized by synapsin-1 staining. Synapses were defined as the overlap of synapsin-1 and spines. The percentage of PTP1B puncta in each condition was calculated.
创建时间：
2012-07-23
