Transcriptomic analysis of BNC2 regulated genes in mouse EMS404 cells. Transcriptomic analysis of BNC2 regulated genes in mouse EMS404 cells

Mouse myofibroblastic EMS404 cells were transfected using a siRNA directed against BNC2 (siBNC2) or a control siRNA (siCTRL) and RNA were processed for microarray analyses. Overall design: Transfection of siRNA (used at 20 nM) into EMS404 cells was performed using lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). siBNC2 (Cat# 4390771 s109765) and Silencer select negative control (Thermo Fisher Scientific Cat# 4390844) were used. 48h after transfection, RNA was purified and processed for analysis using Affymetrix GeneChip arrays (MoGene2.0st) according to standard procedures.