Gene expression changes in galls at 21 dai and corresponding uninfected roots from Poplar CAD and WT lines

Illumina technology was used to generate mRNA profiles of galls from root-knot nematodes infected and corresponding uninfected roots from Poplar CAD and WT lines. RNA was extracted from 3 replicates.TruSeq mRNA Stranded libraries were constructed and after pooling and normalization of libraries, sequencing was done on a NextSeq500 Sequencing System. Raw reads were trimmed for quality and mapped to the substituted genome sequence of P. tremula x P. alba 717-1B4 using CLC Genomics Workbench v9.5.2 and the primary transcripts only. Overall design: mRNA profiles of galls and uninfected roots from Poplar CAD and WT lines were generated by NextSeq500 sequencing (2x75bp). Three biological replicates were sequenced.