Harvested primary hepatocytes exposed to either normoxia or hypoxia (1% O2)

Hepatocytes endogenously express high levels of the enzyme tryptophan dehydrogenase (TDO2), in the current study we explore the effect of reduced oxygen on hepatocyte Tryptophan metabolism Primary hepatoctes were harvested from 8 to 12 week old wildtype C57BL/6 mice and taken into culture, following which they were either cultured under normoxic (19.5%) or hypoxic (1% O2) conditions for 72 hours before harvest for microarray analysis.