Expression data from glioblastoma stem-like cells (GSCs) and astrocyte co-cultured GSCs

consequences of astrocytes on GSCs, gene expression profiles generated from glioblastoma stem-like cells grown alone (mono-culture) and compared to those generated 48h after the initiation of co-culture with astrocytes We used microarrays show that astrocytes are capable to modify via a paracrine mechanism GSC gene expression and thus phenotype. GSCs were seeded into 6-wellplates with astrocytes onto the corresponding trans-well inserts. Co-cultures were initiated by combining the inserts and the 6-well plates; 48h later RNA was collected from GSCs. RNA was collected from three biological replicates was processed and subjected to microarray analysis on Human Genome U133A 2.0 chips. NSC11: glioblastoma stem-like cells isolated from human glioblastoma surgical specimen patient 1 GBAM1: glioblastoma stem-like cells isolated from human glioblastoma surgical specimen patient 2