The IP-MS results of anti-strep and protein A/G in VdΔulpb/Strep-SUMO strain

To identify SUMO-modified proteins and determine the potential SUMO-specific protease target, we first transformed a Strep-VdSUMO construct into VdΔulpb to create the VdΔulpb/Strep-SUMO strain for immunoprecipitation with anti-Strep and protein A/G magnetic beads as negtive control. An anti-Strep-IPed sample was used for mass spectrometry (MS), and twenty-one potential proteins were obtained.