Gut–Lung Axis Perturbation After Spinal Cord Injury 1 and the Probiotic Potential of Bifidobacterium

Paired-end 16S rRNA V3–V4 amplicon reads (PE250, Illumina NovaSeq 6000) from human sputum and mouse lung/gut samples in the SCI study. Demultiplexed FASTQ files with 7-bp sample barcodes; extraction blanks and no-template controls included. The accompanying metadata.tsv provides de-identified sample information (species, tissue, group, time point, treatment), primer sequences (338F/806R), and run identifiers. These data underpin all community analyses (alpha/beta diversity, weighted UniFrac PCoA, PERMANOVA, LEfSe, SourceTracker, random forest). See BioProject IDs PRJNA1100250, PRJNA1100322, PRJNA1100933. No direct identifiers are present.本SCI研究所涉及的人类痰液样本与小鼠肺、肠道样本的双端16S核糖体RNA（16S rRNA）V3-V4区域扩增子测序读段（PE250，Illumina NovaSeq 6000平台）。该数据为已完成去复接的FASTQ文件，带有7碱基样本条形码，包含提取空白对照与无模板对照。配套的metadata.tsv文件提供了去标识化的样本信息（物种、组织、组别、时间点、处理方式）、引物序列（338F/806R）以及测序运行标识符。本数据集支撑所有微生物群落分析，包括α/β多样性分析、加权UniFrac主坐标分析（PCoA）、置换多元方差分析（PERMANOVA）、线性判别分析效应大小（LEfSe）、SourceTracker溯源分析以及随机森林分析。相关BioProject编号为PRJNA1100250、PRJNA1100322及PRJNA1100933。本数据集未包含任何直接可识别的个体信息。