Plant-specific histone residue F41 restricts H3.1 distribution in heterochromatin
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE93223
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The plant specific H3.1F41 plays crucial role in H3.1 genome-wide deposition pattern. We made single and double mutant transgenic lines between histone 3 variants H3.3 and canonical H3.1 in Arabidopsis. The mutated constracts were fused with FLAG tag and transformed into Arabidopsis driven by their endougenous promoter. ChIP seq experiments were did by anti FLAG tag (Sigma (M8823)).
创建时间:
2019-05-15



