Dynamics in zebrafish development define transcriptomic specificity after angiogenesis inhibitor exposure (Sorafenib and Rotenone)

Time-series transcriptomic data of ZFE exposed to the angiogenesis inhibitor SU4312 with exposure start time points at 0 hpf and 24 hpf. Wild-type adult zebrafish (D. rerio, strain OBI/WIK, generation F3) were kept at 26°C in 20 L aquaria with 1-2 fish/L, fed twice a day and on a 14h:10h light dark cycle. Eggs were collected within 1h of spawning, fertilized and normally developed eggs were selected and incubated at 26°C. Eggs for the 24 hpf exposure were stored for at 26°C with 50 eggs per 100 mL of ISO-water, shaken at 75 rpm. Eggs were exposed at 2 hpf or at 24 hpf. 10 embryos were incubated in 4 mL of treatment or control solution for the desired exposure time in 6 mL covered GC vials. Incubation was done at 26°C in a 12h:12h light dark cycle and shaken at 75 rpm. Two vials were pooled as one replicate (total of 20 embryos). For the 0 hpf exposure, data points have 4 replicates and one time point was measured (96 hpf). Embryos were exposed to 2.4 μM Sorafenib and 25 nM Rotenone. For the 24 hpf exposure there 3 time points (36, 48 and 96 hpf), with three replicates per time point and concentration. Two concentrations were measured per substance: 1.3 and 2.4 μM for Sorafenib and 25 and 50 nM for Rotenone.