Table S1 - Identification and Phylogenetic Analysis of <em>Tityus pachyurus</em> and <em>Tityus obscurus</em> Novel Putative Na⁺-Channel Scorpion Toxins

Proposed nomenclature for the 65 members grouped in 14 subfamilies of long-chain NaTxs from Tityus genus scorpions. Gaps were introduced to improve the alignment. Identical residues of each subfamily are shaded in grey. The percentage identity (% Id.) was calculated using ClustalW algorithm (http://www.ebi.ac.uk/Tools/msa/clustalw2/), considering as 100% the toxin which completes primary sequence was firstly described from each subfamily. In the Characteristics column, three parameters were considered. (i) Protein existence: P for the toxins isolated and partially or completely sequenced from the venom; P* for the toxins which were similar to the putative toxins only by molecular mass comparison; T for the putative toxins evidenced at transcript level. (ii) Function: Arthr, Mice, Ins and Frog for toxins active on arthropods, mice, insects or frog nerve, respectively; Antm for antimicrobial peptides; Immun for an immunogenic protein; and Allerg for a peptide which induced generalized allergic reaction on mice. (iii) Classification: α or β for the toxins with electrophysiological data by means of patch clamp techniques; α′ for the toxins with β-toxin structure but pharmacological features of α-toxins; α for the toxin for which the α-activity was proposed by means of sucrose gap experiments. For (ii) and (iii) parameters, the not underlined items are those predicted by sequence similarity, but not experimentally determined. The toxins from Tityus pachyurus (Tpa2, Tpa4, Tpa5, Tpa6, Tpa7 and Tpa8) and Tityus obscurus (Tc49b, Tc48a, Tc48b/Tc49a and To4 to To15) are in bold. Ts1, Ts2, Ts3, Ts5, Ts6, TsNTxP are from T. serrulatus; Tb1, Tb2, Tb2-II, Tb3, TbTx5 and TbIT-1 are from T. bahiensis; Tst1, Tst2 and Tst3 are from T. stigmurus; Tco-gamma and TcoNTxP1 are from T. costatus; Tf4 is from T. fasciolatus; Tz1 and Tz2 are from T. zulianus; and Td1 to Td12, TdNa1, TdNa2, TdNa3, TdNa5, TdNa6, TdNa7, TdNa8, TdNa9, TdNa10, Ardiscretin, Bactridin-1 and Bactridin-2 are from T. discrepans. The new toxins identified or completely sequenced in the present study are identified by traces in the reference column. Partially sequenced toxins were not taken into account. (DOCX)