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Real-time PCR analysis indicated a downregulation of the mRNA levels for ASPN (A), and an increased level of transcripts for BMP-2 (B) after the treatment with 1nm of 1,25(OH)2D3. (C) Correlation between BMP-2 and ASPN on day 14. Experiments were performed in triplicate three times, with comparable results obtained on each occasion. Bars represent mean ± standard deviation (SD) where intergroup analysis statistical differences are indicated by different lowercase letters, and intragroup statistical significant differences are indicated by different uppercase letters. The letter 'Y' represents a difference with respect to day 3. The letter 'Z' represents a difference with respect to day 7. (P < 0.05)