The transcriptome of the marine copepod Temora longicornis under heat stress and recovery. The transcriptome of the marine copepod Temora longicornis under heat stress and recovery

Our manuscript aimed to use RNA sequencing technology to study the transcriptomics responses and mechanisms of this species to temperature fluctuations. We focused on both a short term and a more sustained exposure to heat stress. We report significant effects of temperature on the gene expression of Temora. Overall design: Eight replicates were kept for four days at 14 °C and the other eight were acclimatized to 17 °C over the course of 24 h and kept at this temperature for the remaining three days. Subsequently at day four, four randomly selected replicates were moved for 24 hours to 17 °C from the 14 °C group and vice versa. This resulted in four treatment groups (1) a group exposed to 14°C for five days, (2) a group exposed to 17°C for five days (below referred to as the 'sustained exposure' group), (3) a group exposed to 14°C for four days and then to an additional acute exposure of 17°C (below referred to as 'acute exposure' group), and (4) a group exposed to 17°C for three days and then to an additional recovery exposure of 14°C (below referred to as 'recovery exposure' group). Only 3 replicates for each treatment group were sequenced.